Phylogenetic analysis of and oligonucleotide probe development for Eikelboom type 021N filamentous bacteria isolated from bulking activated sludge

Author(s)
Takahiro Kanagawa, Y Kamagata, Shinobu Aruga, Tetsuro Kohno, Matthias Horn, Michael Wagner
Abstract

Fifteen filamentous strains, morphologically classified as Eikelboom type 021N bacteria, were isolated from bulking activated sludges. Based on comparative 16S ribosomal DNA (rDNA) sequence analysis, all strains form a monophyletic cluster together with all recognized Thiothrix species (88.3 to 98.7% 16S rDNA sequence similarity) within the gamma-subclass of Proteobacteria. The investigated Eikelboom type 021N isolates were subdivided into three distinct groups (I to III) demonstrating a previously unrecognized genetic diversity hidden behind the uniform morphology of the filaments. For in situ detection of these bacteria, 16S rRNA-targeted oligonucleotide probes specific for the entire Eikelboom type 021N-Thiothrix cluster and the Eikelboom type 021N groups I, II, and III, respectively, were designed, evaluated, and successfully applied in activated sludge.

Organisation(s)
External organisation(s)
National Institute of Advanced Industrial Science and Technology (AIST), Nagaoka Institute of Technology
Journal
Applied and Environmental Microbiology
Volume
66
Pages
5043-5052
No. of pages
10
ISSN
0099-2240
Publication date
2000
Peer reviewed
Yes
Austrian Fields of Science 2012
1060 Biology
Portal url
https://ucrisportal.univie.ac.at/en/publications/1989556e-23a9-409d-bc07-5f8ca74357fc