Phylogenetic analysis of and oligonucleotide probe development for Eikelboom type 021N filamentous bacteria isolated from bulking activated sludge
- Author(s)
- Takahiro Kanagawa, Y Kamagata, Shinobu Aruga, Tetsuro Kohno, Matthias Horn, Michael Wagner
- Abstract
Fifteen filamentous strains, morphologically classified as Eikelboom type 021N bacteria, were isolated from bulking activated sludges. Based on comparative 16S ribosomal DNA (rDNA) sequence analysis, all strains form a monophyletic cluster together with all recognized Thiothrix species (88.3 to 98.7% 16S rDNA sequence similarity) within the gamma-subclass of Proteobacteria. The investigated Eikelboom type 021N isolates were subdivided into three distinct groups (I to III) demonstrating a previously unrecognized genetic diversity hidden behind the uniform morphology of the filaments. For in situ detection of these bacteria, 16S rRNA-targeted oligonucleotide probes specific for the entire Eikelboom type 021N-Thiothrix cluster and the Eikelboom type 021N groups I, II, and III, respectively, were designed, evaluated, and successfully applied in activated sludge.
- Organisation(s)
- External organisation(s)
- National Institute of Advanced Industrial Science and Technology (AIST), Nagaoka Institute of Technology
- Journal
- Applied and Environmental Microbiology
- Volume
- 66
- Pages
- 5043-5052
- No. of pages
- 10
- ISSN
- 0099-2240
- Publication date
- 2000
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 1060 Biology
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/1989556e-23a9-409d-bc07-5f8ca74357fc